Deletion and replacement of the mouse adult beta-globin genes by a "plug and socket" repeated targeting strategy.

We describe a two-step strategy to alter any mouse locus repeatedly and efficiently by direct positive selection. Using conventional targeting for the first step, a functional neo gene and a nonfunctional HPRT minigene (the "socket") are introduced into the genome of HPRT- embryonic stem (ES) cells...

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Detalles Bibliográficos

Publicado en:	Mol Cell Biol
Principais autores:	Detloff, P J, Lewis, J, John, S W, Shehee, W R, Langenbach, R, Maeda, N, Smithies, O
Formato:	Artigo
Idioma:	Inglês
Publicado:	Taylor & Francis 1994
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC359224/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7935410/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.14.10.6936-6943.1994
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