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DNA Fragments Assembly Based on Nicking Enzyme System
A couple of DNA ligation-independent cloning (LIC) methods have been reported to meet various requirements in metabolic engineering and synthetic biology. The principle of LIC is the assembly of multiple overlapping DNA fragments by single-stranded (ss) DNA overlaps annealing. Here we present a meth...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3590185/ https://ncbi.nlm.nih.gov/pubmed/23483947 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0057943 |
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