Scarless assembly of unphosphorylated DNA fragments with a simplified DATEL method

Efficient assembly of multiple DNA fragments is a pivotal technology for synthetic biology. A scarless and sequence-independent DNA assembly method (DATEL) using thermal exonucleases has been developed recently. Here, we present a simplified DATEL (sDATEL) for efficient assembly of unphosphorylated...

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Bibliografiske detaljer
Udgivet i:Bioengineered
Main Authors: Ding, Wenwen, Weng, Huanjiao, Jin, Peng, Du, Guocheng, Chen, Jian, Kang, Zhen
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 2017
Fag:
Review CRISPR Special Focus
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5470510/
https://ncbi.nlm.nih.gov/pubmed/28384080
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/21655979.2017.1308986
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