A carregar...
Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA
N.ϕGamma is a strand-specific and site-specific DNA nicking enzyme (YCG↓GT or AC↑CGR). Here we describe the isolation of single and double mutants of N.ϕGamma with attenuated activity. The nicking domains (NDs) of E59A and 11 double mutants were fused to the 5mCG-binding domain of MBD2 and generated...
Na minha lista:
| Main Authors: | , |
|---|---|
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2014
|
| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4027164/ https://ncbi.nlm.nih.gov/pubmed/24609382 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku192 |
| Tags: |
Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
|