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Engineering nicking enzymes that preferentially nick 5-methylcytosine-modified DNA

N.ϕGamma is a strand-specific and site-specific DNA nicking enzyme (YCG↓GT or AC↑CGR). Here we describe the isolation of single and double mutants of N.ϕGamma with attenuated activity. The nicking domains (NDs) of E59A and 11 double mutants were fused to the 5mCG-binding domain of MBD2 and generated...

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Detalhes bibliográficos
Main Authors: Gutjahr, Alice, Xu, Shuang-yong
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4027164/
https://ncbi.nlm.nih.gov/pubmed/24609382
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku192
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