Engineering BspQI Nicking Enzymes and Application of N.BspQI in DNA Labeling and Production of Single-strand DNA

BspQI is a thermostable Type IIS restriction endonuclease (REase) with the recognition sequence 5’ GCTCTTC N1/N4 3’. Here we report the cloning and expression of the bspQIR gene for the BspQI restriction enzyme in E. coli. Alanine scanning of the BspQI charged residues identified a number of DNA nic...

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Main Authors: Zhang, Penghua, Too, Priscilla Hiu-Mei, Samuelson, James C., Chan, Siu-Hong, Vincze, Tamas, Doucette, Stephanie, Bäckström, Stefan, Potamousis, Konstantinos D., Schramm, Timothy M., Forrest, Dan, Schwartz, David C., Xu, Shuang-yong
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2783397/
https://ncbi.nlm.nih.gov/pubmed/19747545
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.09.003
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