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A strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation
BACKGROUND: With the rapid development of synthetic biology, the demand for assembling multiple DNA (genes) fragments into a large circular DNA structure in one step has dramatically increased. However, for constructions of most circular DNA, there are two contradictions in the ligation/assembly and...
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| Publicado no: | Microb Cell Fact |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4455692/ https://ncbi.nlm.nih.gov/pubmed/25896825 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-015-0204-x |
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