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Heavy Enzyme Kinetic Isotope Effects on Proton Transfer in Alanine Racemase
The catalytic effects of perdeuterating the pyridoxal phosphate dependent enzyme alanine racemase from Geobacillus stearothermophilus are reported. The heavy, perdeuterated form is ~5.5% greater in mass than the protiated form, causing kinetic isotope effects (KIEs) of ~1.3 on k(cat) and k(cat)/K(M)...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3579662/ https://ncbi.nlm.nih.gov/pubmed/23373756 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja3101243 |
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