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Heavy Enzyme Kinetic Isotope Effects on Proton Transfer in Alanine Racemase

The catalytic effects of perdeuterating the pyridoxal phosphate dependent enzyme alanine racemase from Geobacillus stearothermophilus are reported. The heavy, perdeuterated form is ~5.5% greater in mass than the protiated form, causing kinetic isotope effects (KIEs) of ~1.3 on k(cat) and k(cat)/K(M)...

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Detaylı Bibliyografya
Asıl Yazarlar: Toney, Michael D., Castro, Joan Nieto, Addington, Trevor A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2013
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3579662/
https://ncbi.nlm.nih.gov/pubmed/23373756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja3101243
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