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Measuring Rates of Ubiquitin Chain Formation as a Functional Readout of Ligase Activity
Specificity within the pathways of ubiquitin conjugation are defined by protein-binding affinities among the components. Enzyme kinetics provides a facile high-resolution experimental approach for quantitating such protein-binding affinities and yields additional mechanistic insights into the transi...
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| Hlavní autoři: | , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2012
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3579653/ https://ncbi.nlm.nih.gov/pubmed/22350887 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-474-2_14 |
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