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Real-time monitoring of tyrosine hydroxylase activity using a plate reader assay

Tyrosine hydroxylase (TH) is the rate-limiting step in dopamine (DA) synthesis, oxidizing tyrosine to l-DOPA, which is further metabolized to DA. Current assays for monitoring activity of this enzyme require extensive work-up, require long analysis time, and measure end points, thereby lacking real-...

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Détails bibliographiques
Auteurs principaux: Vermeer, Lydia M., Higgins, Colin A., Roman, David L., Doorn, Jonathan A.
Format: Artigo
Langue:Inglês
Publié: 2012
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3579528/
https://ncbi.nlm.nih.gov/pubmed/23010244
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.09.005
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