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Expression and purification of recombinant human tyrosine hydroxylase as a fusion protein in Escherichia coli

Tyrosine hydroxylase is the rate-limiting step in the synthesis of dopamine and is tightly regulated. Previous studies have shown it to be covalently modified and potently inhibited by 3,4-dihydroxyphenylacetaldehyde (DOPAL), an endogenous neurotoxin via dopamine catabolism which is relevant to Park...

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Autors principals: Higgins, Colin A., Vermeer, Lydia M., Doorn, Jonathan A., Roman, David L.
Format: Artigo
Idioma:Inglês
Publicat: 2012
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3525113/
https://ncbi.nlm.nih.gov/pubmed/22659380
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2012.05.007
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