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Dual RMCE for efficient re-engineering of mouse mutant alleles
We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. d...
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| Autores principales: | , , , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3576631/ https://ncbi.nlm.nih.gov/pubmed/20953177 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1521 |
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