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Dual RMCE for efficient re-engineering of mouse mutant alleles

We have developed dual recombinase-mediated cassette exchange (dRMCE) to efficiently re-engineer the thousands of available conditional alleles in mouse embryonic stem cells. dRMCE takes advantage of the wild-type loxP and FRT sites present in these conditional alleles and in many gene-trap lines. d...

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Detalhes bibliográficos
Main Authors: Osterwalder, Marco, Galli, Antonella, Rosen, Barry, Skarnes, William C, Zeller, Rolf, Lopez-Rios, Javier
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3576631/
https://ncbi.nlm.nih.gov/pubmed/20953177
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.1521
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