Genetic Incorporation of Twelve meta-Substituted Phenylalanine Derivatives Using A Single Pyrrolysyl-tRNA Synthetase

When coexpressed with its cognate amber suppressing [Formula: see text] , a pyrrolysyl-tRNA synthetase mutant N346A/C348A is able to genetically incorporate twelve meta-substituted phenylalanine derivatives into proteins site-specifically at amber mutation sites in Escherichia coli. These geneticall...

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Main Authors: Wang, Yane-Shih, Fang, Xinqiang, Chen, Hsueh-Ying, Wu, Bo, Wang, Zhiyong U., Hilty, Christian, Liu, Wenshe R.
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3574229/
https://ncbi.nlm.nih.gov/pubmed/23138887
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/cb300512r
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