Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions

This protocol describes an EDTA-based passaging procedure to be used with chemically defined E8 medium that serves as a tool for basic and translational research into human pluripotent stem cells (iPSCs). In this protocol, passaging one six-well or 10 cm plate of cells takes about 6–7 min. This enzy...

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Hlavní autoři: Beers, Jeanette, Gulbranson, Daniel R., George, Nicole, Siniscalchi, Lauren I., Jones, Jeffrey, Thomson, James A., Chen, Guokai
Médium: Artigo
Jazyk:Inglês
Vydáno: 2012
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3571618/
https://ncbi.nlm.nih.gov/pubmed/23099485
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.130
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