Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations

Enzymes used for passaging human pluripotent stem cells (hPSCs) digest cell surface proteins, resulting in cell damage. Moreover, cell dissociation using divalent cation-free solutions causes apoptosis. Here we report that Mg(2+) and Ca(2+) control cell-fibronectin and cell-cell binding of hPSCs, re...

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Bibliografiske detaljer
Main Authors: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K., Asashima, Makoto
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group 2014
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3983606/
https://ncbi.nlm.nih.gov/pubmed/24721898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04646
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