Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations

Enzymes used for passaging human pluripotent stem cells (hPSCs) digest cell surface proteins, resulting in cell damage. Moreover, cell dissociation using divalent cation-free solutions causes apoptosis. Here we report that Mg(2+) and Ca(2+) control cell-fibronectin and cell-cell binding of hPSCs, re...

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Detaylı Bibliyografya
Asıl Yazarlar: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K., Asashima, Makoto
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Nature Publishing Group 2014
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Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3983606/
https://ncbi.nlm.nih.gov/pubmed/24721898
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04646
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