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Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations
Enzymes used for passaging human pluripotent stem cells (hPSCs) digest cell surface proteins, resulting in cell damage. Moreover, cell dissociation using divalent cation-free solutions causes apoptosis. Here we report that Mg(2+) and Ca(2+) control cell-fibronectin and cell-cell binding of hPSCs, re...
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| Main Authors: | , , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3983606/ https://ncbi.nlm.nih.gov/pubmed/24721898 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04646 |
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