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Enzyme-free Passage of Human Pluripotent Stem Cells by Controlling Divalent Cations
Enzymes used for passaging human pluripotent stem cells (hPSCs) digest cell surface proteins, resulting in cell damage. Moreover, cell dissociation using divalent cation-free solutions causes apoptosis. Here we report that Mg(2+) and Ca(2+) control cell-fibronectin and cell-cell binding of hPSCs, re...
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| Hoofdauteurs: | , , , , , , , , , , |
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| Formaat: | Artigo |
| Taal: | Inglês |
| Gepubliceerd in: |
Nature Publishing Group
2014
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| Onderwerpen: | |
| Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3983606/ https://ncbi.nlm.nih.gov/pubmed/24721898 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep04646 |
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