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Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions
This protocol describes an EDTA-based passaging procedure to be used with chemically defined E8 medium that serves as a tool for basic and translational research into human pluripotent stem cells (iPSCs). In this protocol, passaging one six-well or 10 cm plate of cells takes about 6–7 min. This enzy...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3571618/ https://ncbi.nlm.nih.gov/pubmed/23099485 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2012.130 |
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