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Adaptation of Human Pluripotent Stem Cells to Feeder-Free Conditions in Chemically Defined Medium with Enzymatic Single-Cell Passaging

This protocol describes the culture of human pluripotent stem cells (PSCs) under feeder-free conditions in a commercially available, chemically defined, growth medium, using Matrigel as a substrate and the enzyme solution Accutase for single-cell passaging. This system is strikingly different from t...

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Autors principals: Stover, Alexander E., Schwartz, Philip H.
Format: Artigo
Idioma:Inglês
Publicat: 2011
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3674824/
https://ncbi.nlm.nih.gov/pubmed/21822872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-61779-201-4_10
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