Analysis of alterative cleavage and polyadenylation by 3′ region extraction and deep sequencing

Alternative cleavage and polyadenylation (APA) leads to mRNA isoforms with different coding sequences (CDS) and/or 3′ untranslated regions (3′UTRs). Using 3′ Region Extraction And Deep Sequencing (3′READS), a method which addresses the internal priming and oligo(A) tail issues that commonly plague p...

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Detalhes bibliográficos
Main Authors: Hoque, Mainul, Ji, Zhe, Zheng, Dinghai, Luo, Wenting, Li, Wencheng, You, Bei, Park, Ji Yeon, Yehia, Ghassan, Tian, Bin
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3560312/
https://ncbi.nlm.nih.gov/pubmed/23241633
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2288
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