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Complete Mapping of a Cystine Knot and Nested Disulfides of Recombinant Human Arylsulfatase A by Multi-Enzyme Digestion and LC-MS Analysis Using CID and ETD

Cystine knots or nested disulfides are structurally difficult to characterize, despite current technological advances in peptide mapping with high-resolution liquid chromatography coupled with mass spectrometry (LC-MS). In the case of recombinant human arylsulfatase A (rhASA), there is one cystine k...

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書誌詳細
主要な著者: Ni, Wenqin, Lin, Melanie, Salinas, Paul, Savickas, Philip, Karger, Barry L., Wu, Shiaw-Lin
フォーマット: Artigo
言語:Inglês
出版事項: 2012
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC3554868/
https://ncbi.nlm.nih.gov/pubmed/23208745
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13361-012-0510-z
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