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Identification of the Unpaired Cysteine Status and Complete Mapping the 17 Disulfides of Recombinant Tissue Plasminogen Activator Using LC-MS with ETD/CID
Recombinant tissue plasminogen (rt-PA) with 35 cysteine residues has been completely assigned by mapping the 17 disulfide linkages and the unpaired cysteine. The result is consistent with the prediction from homology except for the unassigned cysteine, which was identified at Cys83. This cysteine wa...
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| Autori principali: | , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2010
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2890214/ https://ncbi.nlm.nih.gov/pubmed/20481521 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac100766r |
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