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Simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products using real time PCR-melt curve analysis

The present investigation reports development of post real time PCR (RTi-PCR) - melt curve analysis for simultaneous detection of Listeria monocytogenes and Salmonella spp. The optimal Sybr Green I (SG-I) concentration of 1.6 μM resulted in two specific peaks with melting temperature (Tm) of 79.90 ±...

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Hlavní autoři: Singh, Jitender, Batish, Virender K., Grover, Sunita
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer-Verlag 2011
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3550860/
https://ncbi.nlm.nih.gov/pubmed/23572847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13197-011-0278-3
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