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Simultaneous detection of Listeria monocytogenes and Salmonella spp. in dairy products using real time PCR-melt curve analysis
The present investigation reports development of post real time PCR (RTi-PCR) - melt curve analysis for simultaneous detection of Listeria monocytogenes and Salmonella spp. The optimal Sybr Green I (SG-I) concentration of 1.6 μM resulted in two specific peaks with melting temperature (Tm) of 79.90 ±...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Springer-Verlag
2011
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3550860/ https://ncbi.nlm.nih.gov/pubmed/23572847 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13197-011-0278-3 |
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