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Exploring specific primers targeted against different genes for a multiplex PCR for detection of Listeria monocytogenes

The efficacy of six different sets of primers targeted against 16S rRNA and virulence genes such as ‘iap’, ‘hly’ and ‘prf’ was evaluated in separate PCR assays. The primer pairs targeted against 16S rRNA resulted into amplification of 1.2 kb PCR product. However, sets of primers targeted against dif...

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Bibliografiske detaljer
Udgivet i:3 Biotech
Main Authors: Kumar, Ashwani, Grover, Sunita, Batish, Virender Kumar
Format: Artigo
Sprog:Inglês
Udgivet: Springer Berlin Heidelberg 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4434410/
https://ncbi.nlm.nih.gov/pubmed/28324291
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s13205-014-0225-x
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