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Imaging Protein Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation

We report a new method, Interaction-Dependent PRobe Incorporation Mediated by Enzymes, or ID-PRIME, for imaging protein protein interactions (PPIs) inside living cells. ID-PRIME utilizes a mutant of Escherichia coli lipoic acid ligase, LplA(W37V), which can catalyze the covalent ligation of a coumar...

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Main Authors: Slavoff, Sarah A., Liu, Daniel S., Cohen, Justin D., Ting, Alice Y.
Formato: Artigo
Idioma:Inglês
Publicado: 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3547671/
https://ncbi.nlm.nih.gov/pubmed/22098454
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja206435e
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