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Diels-Alder Cycloaddition for Fluorophore Targeting to Specific Proteins inside Living Cells
The inverse-electron-demand Diels-Alder cycloaddition between trans-cyclooctenes and tetrazines is biocompatible and exceptionally fast. We utilized this chemistry for site-specific fluorescence labeling of proteins on the cell surface and inside living mammalian cells by a two-step protocol. E. col...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3381951/ https://ncbi.nlm.nih.gov/pubmed/22176354 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja209325n |
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