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Monitoring the GAP catalyzed H-Ras GTPase reaction at atomic resolution in real time

The molecular reaction mechanism of the GTPase-activating protein (GAP)-catalyzed GTP hydrolysis by Ras was investigated by time resolved Fourier transform infrared (FTIR) difference spectroscopy using caged GTP (P(3)-1-(2-nitro)phenylethyl guanosine 5′-O-triphosphate) as photolabile trigger. This a...

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Bibliografiske detaljer
Main Authors: Allin, Christoph, Ahmadian, Mohammad Reza, Wittinghofer, Alfred, Gerwert, Klaus
Format: Artigo
Sprog:Inglês
Udgivet: The National Academy of Sciences 2001
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC35414/
https://ncbi.nlm.nih.gov/pubmed/11438727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.131549798
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