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Zinc-finger nuclease mediated disruption of Rag1 in the LEW/Ztm rat

BACKGROUND: Engineered zinc-finger nucleases (ZFN) represented an innovative method for the genome manipulation in vertebrates. ZFN introduced targeted DNA double strand breaks (DSB) and initiated non-homologous end joining (NHEJ) after pronuclear or cytoplasmatic microinjection into zygotes. Result...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Zschemisch, Nils-Holger, Glage, Silke, Wedekind, Dirk, Weinstein, Edward J, Cui, Xiaoxia, Dorsch, Martina, Hedrich, Hans-Jürgen
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3522011/
https://ncbi.nlm.nih.gov/pubmed/23136839
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2172-13-60
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