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Studying protein complexes by the yeast two-hybrid system

Protein complexes are typically analysed by affinity purification and subsequent mass spectrometric analysis. However, in most cases the structure and topology of the complexes remains elusive from such studies. Here we investigate how the yeast two-hybrid system can be used to analyse direct intera...

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Библиографические подробности
Главные авторы: Rajagopala, Seesandra V., Sikorski, Patricia, Caufield, J. Harry, Tovchigrechko, Andrey, Uetz, Peter
Формат: Artigo
Язык:Inglês
Опубликовано: 2012
Предметы:
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC3517932/
https://ncbi.nlm.nih.gov/pubmed/22841565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.07.015
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