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Studying protein complexes by the yeast two-hybrid system

Protein complexes are typically analysed by affinity purification and subsequent mass spectrometric analysis. However, in most cases the structure and topology of the complexes remains elusive from such studies. Here we investigate how the yeast two-hybrid system can be used to analyse direct intera...

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Main Authors: Rajagopala, Seesandra V., Sikorski, Patricia, Caufield, J. Harry, Tovchigrechko, Andrey, Uetz, Peter
格式: Artigo
語言:Inglês
出版: 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3517932/
https://ncbi.nlm.nih.gov/pubmed/22841565
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.07.015
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