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Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins

Yeast two-hybrid (Y2H) screens often produce vastly non-overlapping interaction data when the screens are conducted in different laboratories, or use different vectors, strains, or reporter genes. Here we investigate the underlying reasons for such inconsistencies and compare the effect of 7 differe...

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Bibliografiske detaljer
Main Authors: Rajagopala, Seesandra Venkatappa, Hughes, Kelly T., Uetz, Peter
Format: Artigo
Sprog:Inglês
Udgivet: 2009
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2818629/
https://ncbi.nlm.nih.gov/pubmed/19834901
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pmic.200900282
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