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Mapping the Protein–Protein Interactome Networks Using Yeast Two-Hybrid Screens
The yeast two-hybrid system (Y2H) is a powerful method to identify binary protein–protein interactions in vivo. Here we describe Y2H screening strategies that use defined libraries of open reading frames (ORFs) and cDNA libraries. The array-based Y2H system is well suited for interactome studies of...
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| Publicado no: | Prokaryotic Systems Biology |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7120425/ https://ncbi.nlm.nih.gov/pubmed/26621469 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-3-319-23603-2_11 |
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