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A comparison and optimization of yeast two-hybrid systems

Two-hybrid (Y2H) assays are available in a variety of different versions, including bacterial, yeast, and mammalian systems. However, even when done exclusively in yeast, multiple different host strains, vectors, reporter genes, or protocols can be used. Here we systematically compare protein-protei...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Methods
Hauptverfasser: Caufield, J. Harry, Sakhawalkar, Neha, Uetz, Peter
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2012
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5737776/
https://ncbi.nlm.nih.gov/pubmed/23231818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.12.001
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