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piggyBac Transposon System Modification of Primary Human T Cells

The piggyBac transposon system is naturally active, originally derived from the cabbage looper moth(1,2). This non-viral system is plasmid based, most commonly utilizing two plasmids with one expressing the piggyBac transposase enzyme and a transposon plasmid harboring the gene(s) of interest betwee...

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Main Authors: Saha, Sunandan, Nakazawa, Yozo, Huye, Leslie E., Doherty, Joseph E., Galvan, Daniel L., Rooney, Cliona M., Wilson, Matthew H.
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2012
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3514050/
https://ncbi.nlm.nih.gov/pubmed/23149543
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/4235
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