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Hyperactive piggyBac Gene Transfer in Human Cells and In Vivo

We characterized a recently developed hyperactive piggyBac (pB) transposase enzyme [containing seven mutations (7pB)] for gene transfer in human cells in vitro and to somatic cells in mice in vivo. Despite a protein level expression similar to that of native pB, 7pB significantly increased the gene...

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Detalhes bibliográficos
Main Authors: Doherty, Joseph E., Huye, Leslie E., Yusa, Kosuke, Zhou, Liqin, Craig, Nancy L., Wilson, Matthew H.
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3300075/
https://ncbi.nlm.nih.gov/pubmed/21992617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/hum.2011.138
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