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The Extragenic Spacer Length Between the 5′ and 3′ Ends of the Transgene Expression Cassette Affects Transgene Silencing From Plasmid-based Vectors

In quiescent tissues, minicircle DNA vectors provide at least 10 times higher sustained levels of transgene expression compared to that achieved with a canonical plasmid containing the same expression cassette. It is not known if there is a specific DNA sequence or structure that is needed for DNA s...

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Autori principali: Lu, Jiamiao, Zhang, Feijie, Xu, Siqun, Fire, Andrew Z, Kay, Mark A
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3498813/
https://ncbi.nlm.nih.gov/pubmed/22565847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mt.2012.65
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