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A Simplified Method for the Efficient Refolding and Purification of Recombinant Human GM-CSF

Human granulocyte macrophage colony-stimulating factor (hGM-CSF) is a haematopoietic growth factor and proinflammatory cytokine. Recombinant hGM-CSF is important not only as a research tool but also as a biotherapeutic. However, rhGM-CSF expressed in E. coli is known to form inclusion bodies of misf...

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Autori principali: Thomson, Christy A., Olson, Melanie, Jackson, Linda M., Schrader, John W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2012
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3498172/
https://ncbi.nlm.nih.gov/pubmed/23166789
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0049891
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