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Method for Efficient Refolding and Purification of Chemoreceptor Ligand Binding Domain
Identification of natural ligands of chemoreceptors and structural studies aimed at elucidation of the molecular basis of the ligand specificity can be greatly facilitated by the production of milligram amounts of pure, folded ligand binding domains. Attempts to heterologously express periplasmic li...
Enregistré dans:
| Publié dans: | J Vis Exp |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5755555/ https://ncbi.nlm.nih.gov/pubmed/29286481 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57092 |
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