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Transformation of mammalian cells with an amplifiable dominant-acting gene.
We have transferred a mutant hamster gene coding for an altered dihydrofolate reductase to wild-type cultured mouse cells by using total genomic DNA from methotrexate-resistant Chinese hamster ovary A29 cells as donor. By demonstrating the presence of hamster gene sequences in transformants we have...
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| izdano v: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1980
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349658/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6251468/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.6.3567 |
| Oznake: |
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