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Targeted homologous recombination at the endogenous adenine phosphoribosyltransferase locus in Chinese hamster cells.

We have developed a system that permits analysis of targeted homologous recombination at an endogenous, chromosomal gene locus in cultured mammalian cells. Using a hemizygous, adenine phosphoribosyltransferase (APRT)-deficient, Chinese hamster ovary (CHO) cell mutant as a transfection recipient, we...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Adair, G M, Nairn, R S, Wilson, J H, Seidman, M M, Brotherman, K A, MacKinnon, C, Scheerer, J B
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1989
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC287313/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2734308/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.12.4574
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