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Transformation of mammalian cells with an amplifiable dominant-acting gene.

We have transferred a mutant hamster gene coding for an altered dihydrofolate reductase to wild-type cultured mouse cells by using total genomic DNA from methotrexate-resistant Chinese hamster ovary A29 cells as donor. By demonstrating the presence of hamster gene sequences in transformants we have...

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Bibliografische gegevens
Gepubliceerd in:Proc Natl Acad Sci U S A
Hoofdauteurs: Wigler, M, Perucho, M, Kurtz, D, Dana, S, Pellicer, A, Axel, R, Silverstein, S
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: National Academy of Sciences 1980
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349658/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6251468/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.77.6.3567
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