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Transformation of mammalian cells with an amplifiable dominant-acting gene.

We have transferred a mutant hamster gene coding for an altered dihydrofolate reductase to wild-type cultured mouse cells by using total genomic DNA from methotrexate-resistant Chinese hamster ovary A29 cells as donor. By demonstrating the presence of hamster gene sequences in transformants we have...

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Autores principales: Wigler, M, Perucho, M, Kurtz, D, Dana, S, Pellicer, A, Axel, R, Silverstein, S
Formato: Artigo
Lenguaje:Inglês
Publicado: 1980
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC349658/
https://ncbi.nlm.nih.gov/pubmed/6251468
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