Fluorescence Lifetime Readouts of Troponin-C-Based Calcium FRET Sensors: A Quantitative Comparison of CFP and mTFP1 as Donor Fluorophores

We have compared the performance of two Troponin-C-based calcium FRET sensors using fluorescence lifetime read-outs. The first sensor, TN-L15, consists of a Troponin-C fragment inserted between CFP and Citrine while the second sensor, called mTFP-TnC-Cit, was realized by replacing CFP in TN-L15 with...

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Autores principales: Laine, Romain, Stuckey, Daniel W., Manning, Hugh, Warren, Sean C., Kennedy, Gordon, Carling, David, Dunsby, Chris, Sardini, Alessandro, French, Paul M. W.
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2012
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3494685/
https://ncbi.nlm.nih.gov/pubmed/23152874
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0049200
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