Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM

We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...

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Detalhes bibliográficos
Publicado no:Sensors (Basel)
Main Authors: Chennell, George, Willows, Robin J. W., Warren, Sean C., Carling, David, French, Paul M. W., Dunsby, Chris, Sardini, Alessandro
Formato: Artigo
Idioma:Inglês
Publicado em: MDPI 2016
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5017477/
https://ncbi.nlm.nih.gov/pubmed/27548185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s16081312
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