Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM

We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...

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Detalles Bibliográficos
Publicado en:Sensors (Basel)
Autores principales: Chennell, George, Willows, Robin J. W., Warren, Sean C., Carling, David, French, Paul M. W., Dunsby, Chris, Sardini, Alessandro
Formato: Artigo
Lenguaje:Inglês
Publicado: MDPI 2016
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC5017477/
https://ncbi.nlm.nih.gov/pubmed/27548185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s16081312
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