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Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM

We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase...

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書誌詳細
出版年:Sensors (Basel)
主要な著者: Chennell, George, Willows, Robin J. W., Warren, Sean C., Carling, David, French, Paul M. W., Dunsby, Chris, Sardini, Alessandro
フォーマット: Artigo
言語:Inglês
出版事項: MDPI 2016
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5017477/
https://ncbi.nlm.nih.gov/pubmed/27548185
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/s16081312
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