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Deoxynucleoside [1-thio]triphosphates prevent proofreading during in vitro DNA synthesis.

The contribution of proofreading to the fidelity of catalysis by DNA polymerases has been determined with deoxyribonucleoside [1-thio]triphosphate substrates. These analogues, which contain a sulfur in place of an oxygen on the alpha phosphorus, are incorporated into DNA by DNA polymerases at rates...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Kunkel, T A, Eckstein, F, Mildvan, A S, Koplitz, R M, Loeb, L A
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1981
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349124/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6458818/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.11.6734
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