Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.
Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and RNA polymerases in vitro. Polynucleotides cont...
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| Yayımlandı: | Proc Natl Acad Sci U S A |
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| Asıl Yazarlar: | , , |
| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
National Academy of Sciences
1981
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349103/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6273878/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.11.6633 |
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