Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Analogs of dUTP and UTP that contain a biotin molecule covalently bound to the C-5 position of the pyrimidine ring through an allylamine linker arm have been synthesized. These biotin-labeled nucleotides are efficient substrates for a variety of DNA and RNA polymerases in vitro. Polynucleotides cont...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Langer, P R, Waldrop, A A, Ward, D C
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1981
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC349103/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6273878/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.11.6633
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