Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such biotin-lab...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Kempe, T, Sundquist, W I, Chow, F, Hu, S L
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 1985
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC340973/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2582352/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/13.1.45
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