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Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.
Biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. The 5'-biotinylated oligonucleotides were enzymatically coupled to other DNA fragments by T4 DNA ligase or T4 RNA ligase. The hybridization properties of such bioti...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
1985
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| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC340973/ https://ncbi.nlm.nih.gov/pubmed/2582352 |
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